제품 > 항체 > 다중클론항체(pAb)

VCAM1 Rabbit pAb (A0279)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HUVEC cells, using VCAM1 Rabbit pAb (A0279) at 1:500 dilution. HUVEC cells were treated by Human Tumor Necrosis Factor-α (hTNF-α) (10 ng/ml) for 16 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from Mouse liver, using VCAM1 Rabbit pAb (A0279) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - VCAM1 Rabbit pAb (A0279)

Immunofluorescence analysis of Human spleen tissue using VCAM1 Rabbit pAb (A0279) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Overview

Product name	VCAM1 Rabbit pAb
Catalog No.	A0279
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1).
Sequence	NRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVEL
Gene ID
Swiss Prot
Synonyms	CD106; INCAM-100; VCAM1
Calculated MW	81kDa
Observed MW	95-120kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HUVEC treated by hTNF-α, Mouse liver
Cellular location	Membrane, Single-pass type I membrane protein.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Cynoglossus semilaevis) IHC(Mus musculus) IF(Mus musculus, Rattus norvegicus) WB(Mus musculus,Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

VCAM1 Rabbit pAb images

Western blot - VCAM1 Rabbit pAb (A0279)

Immunofluorescence - VCAM1 Rabbit pAb (A0279)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

International Journal of Biological Sciences

Application:

IF:

6.58

Species:

Mus musculus

PMID:

26722219

Title:

Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MAPK and NF-κB and Activating HO-1/Nrf2 Pathways

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Cell and bioscience

Application:

IF:

7.133

Species:

Rattus norvegicus

PMID:

28596825

Title:

Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Life sciences

Application:

IF:

5.037

Species:

Homo sapiens

PMID:

30081006

Title:

Astragaloside IV improves vascular endothelial dysfunction by inhibiting the TLR4/NF-κB signaling pathway

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

3.293

Species:

Cynoglossus semilaevis

PMID:

30827402

Title:

In vivo cellular and molecular study on duck spleen infected by duck Tembusu virus.

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Experimental and therapeutic medicine

Application:

IF:

2.447

Species:

Rattus norvegicus

PMID:

31258688

Title:

Lycium barbarum polysaccharide attenuates cardiac hypertrophy, inhibits calpain-1 expression and inhibits NF-κB activation in streptozotocin-induced diabetic rats.

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Neuroscience

Application:

IF:

3.59

Species:

Mus musculus

PMID:

32081723

Title:

Sleep-restriction Inhibits Neurogenesis Through Decreasing the Infiltration of CD169+ Macrophages to Ischemic Brain After Stroke

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

32508661

Title:

Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Mol Med Rep

Application:

IF:

2.95

Species:

Homo sapiens

PMID:

33786624

Title:

Sacubitril/valsartan inhibits ox‑LDL‑induced MALAT1 expression, inflammation and apoptosis by suppressing the TLR4/NF‑κB signaling pathway in HUVECs

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

FASEB J

Application:

IF:

5.191

Species:

Mus musculus

PMID:

34516679

Title:

Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Acta pharmacologica Sinica

Application:

IHC

IF:

7.16

Species:

Mus musculus

PMID:

37041228

Title:

Single-cell RNA sequencing deciphers the mechanism of sepsis-induced liver injury and the therapeutic effects of artesunate

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

6.37

Species:

Mus musculus

PMID:

37528397

Title:

HOXA1 participates in VSMC-to-macrophage-like cell transformation via regulation of NF-κB p65 and KLF4: a potential mechanism of atherosclerosis pathogenesis - PMC

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Cells

Application:

IF:

7.66

Species:

Mus musculus

PMID:

37508502

Title:

Chemokine/ITGA4 Interaction Directs iPSC-Derived Myogenic Progenitor Migration to Injury Sites in Aging Muscle for Regeneration - PMC

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Cancer Research

Application:

IF:

13.312

Species:

Homo sapiens

PMID:

29066510

Title:

Evidence for Kaposi's Sarcoma originating from?Mesenchymal Stem Cell through KSHV-induced Mesenchymal-to-Endothelial Transition

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IF:

11.09

Species:

Mus musculus

PMID:

37883686

Title:

An erythrocyte membrane-derived nanosystem for efficient reversal of endothelial injury in sepsis

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

American journal of respiratory cell and molecular biology

Application:

IF:

7.74

Species:

Homo sapiens

PMID:

37071849

Title:

Local Complement Contributes to Pathogenic Activation of Lung Endothelial Cells in SARS-CoV-2 Infection

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.6

Species:

Homo sapiens

PMID:

37771765

Title:

Metabolite Neu5Ac triggers SLC3A2 degradation promoting vascular endothelial ferroptosis and aggravates atherosclerosis progression in ApoE-/-mice - PMC

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.85

Species:

Homo sapiens

PMID:

38331012

Title:

Multifaceted antineoplastic curative potency of novel water-soluble methylimidazole-based oxidovanadium (IV) complex against triple negative mammary carcinoma

References for VCAM1 Rabbit pAb (A0279)

Product:

VCAM1 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Homo sapiens

PMID:

38269270