제품 > 항체 > 다중클론항체(pAb)

VEGF Receptor 2 Rabbit pAb (A5609)

Reviews (3)Publications (19) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of lysates from Mouse heart, using VEGF Receptor 2 Rabbit pAb (A5609) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with VEGF Receptor 2 using VEGF Receptor 2 Rabbit pAb (A5609) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 10s.

Review
ABclonal: review for VEGF Receptor 2 Rabbit pAb(A5609)
Review
ABclonal: review for VEGF Receptor 2 Rabbit pAb(A5609)
Review
ABclonal: review for VEGF Receptor 2 Rabbit pAb(A5609)

Overview

Product nameVEGF Receptor 2 Rabbit pAb
Catalog No.A5609
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1150-1250 of human VEGF Receptor 2 (NP_002244.1).
SequenceRPTFSELVEHLGNLLQANAQQDGKDYIVLPISETLSMEEDSGLSLPTSPVSCMEEEEVCDPKFHYDNTAGISQYLQNSKRKSRPVSVKTFEDIPLEEPEVK
Gene ID
Swiss Prot
SynonymsFLK1; CD309; VEGFR; VEGFR2; VEGF Receptor 2
Calculated MW152kDa
Observed MW210kDa/230kDa/230kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse heart, 293F cells transfected with VEGF Receptor 2
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein.
Customer validation

(Eoma cell, HUVEC cells)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Homo sapiens, Gallus gallus)

IF(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Homo sapiens)

IF(Homo sapiens, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609)}

Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of lysates from Mouse heart, using VEGF Receptor 2 Rabbit pAb (A5609) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609)}

Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with VEGF Receptor 2 using VEGF Receptor 2 Rabbit pAb (A5609) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (5)

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WBELISA

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ELISAWB

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Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Phospho-VEGF Receptor 2-Y1175 Rabbit mAb
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WBELISA

Reactivity:Human

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Human VEGFR2 Monoclonal Antibody
YR0132

ELISAneutralizationfunctionalassayssuchasbioanalyticalPKandADAassaysandthoseassaysforstudyingbiologicalpathways

Reactivity:<1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay

Human VEGFR2 Monoclonal Antibody

ELISA 키트 (1)

Human VEGFR2/Flk-1 ELISA Kit
RK00349

Human VEGFR2/Flk-1 ELISA Kit

재조합 단백질 (3)

Recombinant Human VEGFR-2/KDR/CD309 Protein
RP00084

Tags:C-His

Expressed Host:HEK293 cells

Recombinant Human VEGFR-2/KDR/CD309 Protein

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Tags:C-hFc&His

Expressed Host:HEK293 cells

Recombinant Human VEGFR-2/KDR/CD309 Protein

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Tags:C-His&Avi

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Biotinylated Recombinant Human VEGFR-2/KDR/CD309 Protein

제품 (3)

Rabbit anti-Human VEGFR2/Flk-1 mAb
RMK0020

Rabbit anti-Human VEGFR2/Flk-1 mAb

Rabbit anti-Human VEGFR2/Flk-1 mAb
RMK0019

Rabbit anti-Human VEGFR2/Flk-1 mAb

Rabbit anti-Human VEGFR2/Flk-1 mAb
RMK0391

Rabbit anti-Human VEGFR2/Flk-1 mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)