제품 > 항체 > 다중클론항체(pAb)

Vimentin Rabbit pAb (A2584)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Vimentin Rabbit pAb (A2584) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Western blot analysis of various lysates using Vimentin Rabbit pAb (A2584) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from A-549 cells using Vimentin Rabbit pAb (A2584) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Vimentin Rabbit pAb (A2584)

Immunohistochemistry analysis of paraffin-embedded Human lung using Vimentin Rabbit pAb (A2584) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Vimentin Rabbit pAb (A2584) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Vimentin Rabbit pAb (A2584) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Vimentin Rabbit pAb (A2584)

Confocal immunofluorescence analysis of U2OS cells using Vimentin Rabbit pAb (A2584) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Vimentin Rabbit pAb
Catalog No.	A2584
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin (NP_003371.2).
Sequence	MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAE
Gene ID
Swiss Prot
Synonyms	CTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW	54kDa
Observed MW	58kDa/57kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	K-562, HeLa, Jurkat, NIH/3T3, A-549
Cellular location	Cytoplasm.
Customer validation	(A549, HeLa) IHC(Homo sapiens, Mus musculus, Rattus norvegicus) WB(Mus musculus, Homo sapiens, Mus musculus) IF(Mus musculus, Rattus norvegicus) IF(Homo sapiens, Mus musculus)

Documents

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Research Area

Vimentin Rabbit pAb images

Western blot - Vimentin Rabbit pAb (A2584)

Immunohistochemistry - Vimentin Rabbit pAb (A2584)

Immunofluorescence - Vimentin Rabbit pAb (A2584)

Confocal immunofluorescence analysis of U2OS cells using Vimentin Rabbit pAb (A2584) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2584

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Reviews List

Product Protocols

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Endocrine-Related Cancer

Application:

IHC

IF:

5.678

Species:

Homo sapiens

PMID:

28389503

Title:

The purine receptor P2X7R regulates the release of pro-inflammatory cytokines in human craniopharyngioma

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Cell death & disease

Application:

IHC

IF:

8.469

Species:

Mus musculus

PMID:

30770793

Title:

LXRα promotes cell metastasis by regulating the NLRP3 inflammasome in renal cell carcinoma.

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.575

Species:

Mus musculus

PMID:

32505342

Title:

NLRC5 deficiency ameliorates cardiac fibrosis in diabetic cardiomyopathy by regulating EndMT through Smad2/3 signaling pathway

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Front Oncol

Application:

IF:

6.244

Species:

Homo sapiens

PMID:

33680908

Title:

Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Exp Eye Res

Application:

IF:

3.467

Species:

Homo sapiens

PMID:

33582182

Title:

AQP5 regulates vimentin expression via miR-124-3p.1 to protect lens transparency

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Sci Rep

Application:

IF:

4.379

Species:

Mus musculus

PMID:

34158600

Title:

Photobiomodulation has rejuvenating effects on aged bone marrow mesenchymal stem cells

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Technol Cancer Res Treat

Application:

IF:

3.399

Species:

Homo sapiens

PMID:

34657484

Title:

Influence of Autophagy Inhibition on Lung Adenocarcinoma Cell Migration and Invasion Ability, and Efficacy of Gefitinib

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Mol Cell Biol

Application:

IF:

4.27

Species:

Mus musculus

PMID:

34842443

Title:

Inhibition of SGLT1 alleviates the glycemic variability-induced cardiac fibrosisvia inhibiting activations of macrophage and cardiac fibroblast

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Tissue & cell

Application:

IF:

2.58

Species:

Homo sapiens

PMID:

36156375

Title:

CCL26 silence represses colon cancer by inhibiting the EMT signaling pathway

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

International journal of medical sciences

Application:

IF:

3.64

Species:

Homo sapiens

PMID:

36619219

Title:

TRPM2 facilitates tumor progression of clear cell renal cell carcinoma by relieving Endoplasmic Reticulum Stress - PMC

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Ear, nose, & throat journal

Application:

IF:

1.67

Species:

Homo sapiens

PMID:

36576436

Title:

Silencing PARP-1 binding protein Inhibits Cell Migration and Invasion via Suppressing UBE2C in Nasopharyngeal Cancer Cells

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Translational research : the journal of laboratory and clinical medicine

Application:

IHC

IF:

10.17

Species:

Rattus norvegicus

PMID:

37257560

Title:

Norcantharidin potentiates sorafenib antitumor activity in hepatocellular carcinoma rat model through inhibiting IL-6/STAT3 pathway

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Life sciences

Application:

IHC

IF:

6.78

Species:

Rattus norvegicus

PMID:

37142088

Title:

Carvacrol enhances anti-tumor activity and mitigates cardiotoxicity of sorafenib in thioacetamide-induced hepatocellular carcinoma model through inhibiting TRPM7

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

37072408

Title:

PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer - PMC

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

International journal of immunopathology and pharmacology

Application:

IF:

3.29

Species:

Homo sapiens

PMID:

37864517

Title:

PKNOX1 acts as a transcription factor of DHH and promotes the progression of stomach adenocarcinoma by regulating the Hedgehog signalling pathway - PMC

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Oncotarget

Application:

IHC, IF

IF:

3.765

Species:

Homo sapiens

PMID:

27409178

Title:

TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

Aging-us

Application:

IF:

5.955

Species:

Homo sapiens, Mus musculus

PMID:

31881015

Title:

Hsa_circ_0006948 enhances cancer progression and epithelial-mesenchymal transition through the miR-490-3p/HMGA2 axis in esophageal squamous cell carcinoma.

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

American journal of translational research

Application:

IF:

3.94

Species:

Rattus norvegicus

PMID:

37560208

Title:

The molecular link between obstructive sleep apnea and osteoarthritis: based on bioinformatics analysis and experimental validation - PMC

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

38092315

Title:

Imperatorin ameliorates kidney injury in diabetic mice by regulating the TGF-/Smad2/3 signaling axis, epithelial-to-mesenchymal transition, and renal inflammation

References for Vimentin Rabbit pAb (A2584)

Product:

Vimentin Rabbit pAb

Journal:

BMC cancer

Application:

IF:

3.8

Species:

Mus musculus

PMID:

38388902