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WNK3 Rabbit mAb (A23551)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WNK3 Rabbit mAb (A23551)

Western blot analysis of lysates from HAP1 cells, using WNK3 Rabbit mAb (A23551) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - WNK3 Rabbit mAb (A23551)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using WNK3 Rabbit mAb (A23551) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - WNK3 Rabbit mAb (A23551)

Immunohistochemistry analysis of paraffin-embedded Rat brain using WNK3 Rabbit mAb (A23551) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameWNK3 Rabbit mAb
Catalog No.A23551
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60764
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1700-1800 of human WNK3. (NP_065973.2).
SequenceTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK
Gene ID
Swiss Prot
SynonymsPRKWNK3; WNK3
Calculated MW198kDa
Observed MW170kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHAP1
Cellular locationCytoplasm

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - WNK3 Rabbit mAb (A23551)}

    Western blot - WNK3 Rabbit mAb (A23551)

    Western blot analysis of lysates from HAP1 cells, using WNK3 Rabbit mAb (A23551) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - WNK3 Rabbit mAb (A23551)}

    Immunohistochemistry - WNK3 Rabbit mAb (A23551)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using WNK3 Rabbit mAb (A23551) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - WNK3 Rabbit mAb (A23551)}

    Immunohistochemistry - WNK3 Rabbit mAb (A23551)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using WNK3 Rabbit mAb (A23551) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)