제품 > 항체 > 다중클론항체(pAb)

YTHDC2 Rabbit pAb (A15004)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YTHDC2 Rabbit pAb (A15004)

Western blot analysis of various lysates using YTHDC2 Rabbit pAb (A15004) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameYTHDC2 Rabbit pAb
Catalog No.A15004
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1131-1430 of human YTHDC2 (NP_073739.3).
SequenceHSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSSADTEFSDECTTAERVLMKSPSPALHPPQKYKDRGILHPKRGTEDRSDQSSLKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD
Gene ID
Swiss Prot
SynonymsCAHL; hYTHDC2; YTHDC2
Calculated MW160kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse testis, Rat testis
Cellular locationendoplasmic reticulum, nucleus, perinuclear region of cytoplasm
Customer validation

WB(Homo sapiens, Rana amurensis Boulenger, Mus musculus, Sus scrofa)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - YTHDC2 Rabbit pAb (A15004)}

Western blot - YTHDC2 Rabbit pAb (A15004)

Western blot analysis of various lysates using YTHDC2 Rabbit pAb (A15004) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A15004? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

[KD Validated] YTHDC2 Rabbit pAb
A24219

ELISAWBIF/ICCIP

Reactivity:Human, Mouse, Rat

[KD Validated] YTHDC2 Rabbit pAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)