제품 > 항체 > 다중클론항체(pAb)

c-Fos Rabbit pAb (A0236)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using c-Fos Rabbit pAb (A0236) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from HeLa cells, using c-Fos Rabbit pAb (A0236) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - c-Fos Rabbit pAb (A0236)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg c-Fos antibody (A0236). Western blot was performed from the immunoprecipitate using c-Fos antibody (A0236) at a dilution of 1:1000.

Overview

Product name	c-Fos Rabbit pAb
Catalog No.	A0236
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human c-Fos (NP_005243.1).
Sequence	MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDFCTDLAVSSANFIPTVTAISTSPDLQWLVQPALVSSVAPSQTRAPHPFGVPAPSAGAYSRAGVVKT
Gene ID
Swiss Prot
Synonyms	p55; AP-1; C-FOS; c-Fos
Calculated MW	41kDa
Observed MW	55-60kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	HeLa treated by TPA
Cellular location	Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus, Capra hircus) IHC(Rattus norvegicus, Cervus nippon, Mus musculus) IF(Mus musculus, Mus musculus ) qPCR(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

c-Fos Rabbit pAb images

Western blot - c-Fos Rabbit pAb (A0236)

Immunoprecipitation - c-Fos Rabbit pAb (A0236)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

28842554

Title:

CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Behavioural brain research

Application:

IF:

3.332

Species:

Rattus norvegicus

PMID:

30703397

Title:

Metformin administration prevents memory impairment induced by hypobaric hypoxia in rats.

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Clinical and experimental immunology

Application:

IHC

IF:

4.33

Species:

Rattus norvegicus

PMID:

31344254

Title:

Transcriptomes in rat sciatic nerves at different stages of experimental autoimmune neuritis determined by RNA sequencing.

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.81

Species:

Mus musculus

PMID:

32194406

Title:

MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Open Biology

Application:

IF:

6.411

Species:

Homo sapiens

PMID:

27358292

Title:

Identification of ubiquinol cytochrome c reductase hinge (UQCRH) as a potential diagnostic biomarker for lung adenocarcinoma.

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Drug Des Devel The

Application:

IF:

4.162

Species:

Mus musculus

PMID:

32764870

Title:

N-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264. 7 Cell Osteoclastogenesis

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

J Photochem Photobiol B

Application:

IF:

6.252

Species:

Mus musculus

PMID:

33316624

Title:

The role of FOS-mediated autophagy activation in the indocyanine green-based photodynamic therapy for treating melanoma

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Mol Carcinog

Application:

IF:

4.784

Species:

Homo sapiens

PMID:

34062009

Title:

miR‐506‐loaded gelatin nanospheres target PENK and inactivate the ERK/Fos signaling pathway to suppress triple‐negative breast cancer aggressiveness

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Cell Signal

Application:

IF:

4.315

Species:

Mus musculus

PMID:

34469786

Title:

Downregulated fat mass and obesity-associated protein inhibits bone resorption and osteoclastogenesis by nuclear factor-kappa B inactivation

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Rattus norvegicus

PMID:

34803683

Title:

Hydroxy-Safflower Yellow A Alleviates Osteoporosis in Ovariectomized Rat Model by Inhibiting Carbonic Anhydrase 2 Activity

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

RSC advances

Application:

IF:

4.03

Species:

Mus musculus

PMID:

35497169

Title:

CpG oligodeoxynucleotides inhibit the proliferation and osteoclastic differentiation of RAW264. 7 cells

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Rattus norvegicus

PMID:

35814196

Title:

Echinacoside Inhibits Osteoclast Function by Down-Regulating PI3K/Akt/C-Fos to Alleviate Osteolysis Caused by Periprosthetic Joint Infection - PMC

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Cell regeneration (London, England)

Application:

IHC

IF:

Species:

Cervus nippon

PMID:

36542206

Title:

Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Human gene therapy

Application:

IF:

4.79

Species:

Homo sapiens

PMID:

36373826

Title:

HIV Tat-conjugated Histone H3 peptides induce tumor cell death via cellular stress responses

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

7.52

Species:

Rattus norvegicus

PMID:

37095518

Title:

LOX overexpression programming mediates the osteoclast mechanism of low peak bone mass in female offspring rats caused by pregnant dexamethasone exposure - PMC

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

PeerJ

Application:

IF:

3.06

Species:

Homo sapiens, Mus musculus

PMID:

36890868

Title:

CYP27A1 deficiency promoted osteoclast differentiation - PMC

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Environmental toxicology

Application:

IF:

4.1

Species:

Mus musculus

PMID:

37195267

Title:

METTL14 represses osteoclast formation to ameliorate osteoporosis via enhancing GPX4 mRNA stability

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Atherosclerosis

Application:

IHC

IF:

6.84

Species:

Mus musculus

PMID:

37549548

Title:

LncRNA RP4-639F20. 1 interacts with THRAP3 to attenuate atherosclerosis by regulating c-FOS in vascular smooth muscle cells proliferation and migration

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Brain sciences

Application:

IF:

3.33

Species:

Capra hircus

PMID:

37371401

Title:

Electroacupuncture Relieves Visceral Hypersensitivity via Balancing PAR2 and PAR4 in the Descending Pain Modulatory System of Goats - PMC

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Journal of virology

Application:

WB, IF

IF:

6.54

Species:

Mus musculus

PMID:

36005758

Title:

Lab-Attenuated Rabies Virus Facilitates Opening of the Blood-Brain Barrier by Inducing Matrix Metallopeptidase 8

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

The Journal of nutritional biochemistry

Application:

WB, qPCR

IF:

6.11

Species:

Rattus norvegicus

PMID:

37209954

Title:

Sesamol improves bone mass in ovary intact growing and adult rats but accelerates bone deterioration in the ovariectomized rats

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Acta pharmacologica Sinica

Application:

WB, IF

IF:

7.16

Species:

Mus musculus

PMID:

35948752

Title:

Roxadustat alleviates nitroglycerin-induced migraine in mice by regulating HIF-1/NF-B/inflammation pathway

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Mus musculus

PMID:

36985786

Title:

Ethanol Extract of Rosa laevigata Michx. Fruit Inhibits Inflammatory Responses through NF-B/MAPK Signaling Pathways via AMPK Activation in RAW 264.7 Macrophages - PMC

References for c-Fos Rabbit pAb (A0236)

Product:

c-Fos Rabbit pAb

Journal:

Food & function

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38506303