제품 > 항체 > 다중클론항체(pAb)

eIF2α Rabbit pAb (A0764)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using eIF2α Rabbit pAb (A0764) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review

Overview

Product name	eIF2α Rabbit pAb
Catalog No.	A0764
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human eIF2α (NP_004085.1).
Sequence	MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKE
Gene ID
Swiss Prot
Synonyms	EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF2α
Calculated MW	36kDa
Observed MW	36kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	MCF7, NIH/3T3, PC-12
Cellular location	Cytoplasmic granule.
Customer validation	(293T cells) WB(Mus musculus, Rattus norvegicus, Homo sapiens, Sus scrofa, Chlorocebus aethiops, Gallus gallus, Mus musculus) IF(Mus musculus) IP(Homo sapiens) Other(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

eIF2α Rabbit pAb images

Western blot - eIF2α Rabbit pAb (A0764)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0764

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Cell reports

Application:

IF:

9.423

Species:

Mus musculus

PMID:

30673611

Title:

Gain-of-Function Mutations of SLC16A11 Contribute to the Pathogenesis of Type 2 Diabetes.

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

6.543

Species:

Rattus norvegicus

PMID:

30984338

Title:

Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Cell

Application:

IF:

41.582

Species:

Homo sapiens

PMID:

31031002

Title:

Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity.

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Redox Biology

Application:

IF:

11.799

Species:

Mus musculus

PMID:

30292945

Title:

Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis.

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Applied and Environmental Microbiology

Application:

IF:

4.792

Species:

Homo sapiens

PMID:

30979838

Title:

Commensal Escherichia coli Aggravates Acute Necrotizing Pancreatitis through Targeting of Intestinal Epithelial Cells.

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Toxicology Letters

Application:

IF:

4.372

Species:

Mus musculus

PMID:

33137417

Title:

Adiponectin protects HL-1 cardiomyocytes against rotenone-induced cytotoxicity through AMPK activation

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Front Immunol

Application:

IF:

7.561

Species:

Homo sapiens

PMID:

33133097

Title:

Seneca Valley Virus 3C Protease Inhibits Stress Granule Formation by Disrupting eIF4GI-G3BP1 Interaction

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Front Cell Dev Biol

Application:

IF:

6.684

Species:

Rattus norvegicus

PMID:

34805156

Title:

Eicosapentaenoic Acid-Induced Autophagy Attenuates Intervertebral Disc Degeneration by Suppressing Endoplasmic Reticulum Stress, Extracellular Matrix Degradation, and Apoptosis

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Sus scrofa

PMID:

36035212

Title:

Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

3.24

Species:

Chlorocebus aethiops

PMID:

33360915

Title:

Porcine epidemic diarrhea virus infections induce autophagy in Vero cells via ROS-dependent endoplasmic reticulum stress through PERK and IRE1 pathways

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

35963197

Title:

Piperine alleviates acute pancreatitis: A possible role for FAM134B and CCPG1 dependent ER-phagy

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Communications biology

Application:

IF:

6.54

Species:

Homo sapiens

PMID:

35970927

Title:

Long non-coding RNA DARS-AS1 promotes tumor progression by directly suppressing PACT-mediated cellular stress - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Drug development research

Application:

IF:

Species:

Homo sapiens

PMID:

36598070

Title:

Thrombospondin‐1 mimic peptide PKHB1 induced endoplasmic reticulum stress‐mediated but CD47‐independent apoptosis in non‐small cell lung cancer

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Environmental science and pollution research international

Application:

IF:

5.19

Species:

Gallus gallus

PMID:

36810819

Title:

Selenium alleviates endoplasmic reticulum calcium depletion-induced endoplasmic reticulum stress and apoptosis in chicken myocardium after mercuric chloride …

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

The American journal of Chinese medicine

Application:

IF:

Species:

Rattus norvegicus

PMID:

36458485

Title:

Gardenia jasminoides Extract GJ-4 Alleviates Memory Deficiency of Vascular Dementia in Rats through PERK-Mediated Endoplasmic Reticulum Stress Pathway

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Veterinary research

Application:

IF:

3.82

Species:

Chlorocebus aethiops

PMID:

36949543

Title:

The novel Nsp9-interacting host factor H2BE promotes PEDV replication by inhibiting endoplasmic reticulum stress-mediated apoptosis - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

37316499

Title:

SMURF1 attenuates endoplasmic reticulum stress by promoting the degradation of KEAP1 to activate NRF2 antioxidant pathway - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Sus scrofa

PMID:

37031286

Title:

The apoptotic effects of soybean agglutinin were induced through three different signal pathways by down-regulating cytoskeleton proteins in IPEC-J2 cells - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Cells

Application:

IF:

7.666

Species:

Homo sapiens, Mus musculus

PMID:

34207793

Title:

iPLA2 Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

38377149

Title:

The NP protein of Newcastle disease virus dictates its oncolytic activity by regulating viral mRNA translation efficiency - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IF:

Species:

Sus scrofa

PMID:

38584279

Title:

Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Journal of cellular physiology

Application:

WB, IF

IF:

6.51

Species:

Mus musculus

PMID:

35938526

Title:

Cold exposureinduced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Bioscience, biotechnology, and biochemistry

Application:

IF:

2.33

Species:

Rattus norvegicus

PMID:

34601561

Title:

SMYD1 alleviates septic myocardial injury by inhibiting endoplasmic reticulum stress

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

RSC advances

Application:

IF:

3.9

Species:

Mus musculus,Sus scrofa

PMID:

38495986

Title:

Silica-coated LiYF 4: Yb 3+, Tm 3+ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Developmental cell

Application:

IF:

11.8

Species:

Chlorocebus aethiops

PMID:

38569547

Title:

Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

PLoS pathogens

Application:

IP, Other

IF:

6.7

Species:

Homo sapiens

PMID:

38935808

Title:

The NS2B-PP1-eIF2 axis: Inhibiting stress granule formation and Boosting Zika virus replication

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Behavioural brain research

Application:

IF:

2.7

Species:

Mus musculus

PMID:

38942086

Title:

PERK inhibitor (ISRIB) improves depression-like behavior by inhibitions of HPA-axis over-activation in mice exposed to chronic restraint stress

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

Species:

Mus musculus

PMID:

39191723

Title:

TRPA1 aggravates osteoclastogenesis and osteoporosis through activating endoplasmic reticulum stress mediated by SRXN1 - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Communications biology

Application:

IF:

5.9

Species:

Mus musculus

PMID:

39191965

Title:

Deficiency of flavin-containing monooxygenase 3 protects kidney function after ischemia-reperfusion in mice - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

eLife

Application:

IF:

7.7

Species:

Mus musculus

PMID:

39269275

Title:

TMC7 deficiency causes acrosome biogenesis defects and male infertility in mice - PMC

References for eIF2α Rabbit pAb (A0764)

Product:

eIF2α Rabbit pAb

Journal:

Emerging microbes & infections

Application:

IF:

13.2

Species:

Homo sapiens

PMID:

39230190

Title:

African swine fever virus modulates the endoplasmic reticulum stress-ATF6-calcium axis to facilitate viral replication - PMC

Publishing research using A0764? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

Secondary Antibodies (26)

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제품 > 항체 > 다중클론항체(pAb)

eIF2α Rabbit pAb (A0764)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

eIF2α Rabbit pAb images

Submit an review for A0764

Reviews List

Product Protocols

항체 (4)

Secondary Antibodies (26)