제품 > 항체 > 다중클론항체(pAb)

eNOS Rabbit pAb (A1548)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using eNOS antibody (A1548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - eNOS Rabbit pAb (A1548)

Immunofluorescence analysis of human placenta cells using eNOS Rabbit pAb (A1548) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	eNOS Rabbit pAb
Catalog No.	A1548
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1203 of human eNOS (NP_000594.2).
Sequence	RLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP
Gene ID
Swiss Prot
Synonyms	eNOS; ECNOS
Calculated MW	133kDa
Observed MW	140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Rat placenta, Rat heart
Cellular location	Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton
Customer validation	WB(Rattus norvegicus, Homo sapiens, Mus musculus) IHC(Homo sapiens) IF(Homo sapiens, Rattus norvegicus)

Documents

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Research Area

eNOS Rabbit pAb images

Western blot - eNOS Rabbit pAb (A1548)

Immunofluorescence - eNOS Rabbit pAb (A1548)

Immunofluorescence analysis of human placenta cells using eNOS Rabbit pAb (A1548) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Redox biology

Application:

IF:

11.799

Species:

Rattus norvegicus

PMID:

28734243

Title:

Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Life sciences

Application:

IF:

5.037

Species:

Rattus norvegicus

PMID:

31323271

Title:

Astragaloside IV protects against hyperglycemia-induced vascular endothelial dysfunction by inhibiting oxidative stress and Calpain-1 activation.

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

International Journal of Molecular Sciences

Application:

IF:

5.923

Species:

Homo sapiens

PMID:

31533227

Title:

Protective Effects and Mechanisms of Vaccarin on Vascular Endothelial Dysfunction in Diabetic Angiopathy.

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Mus musculus

PMID:

33014270

Title:

Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Cell Biol Int

Application:

IF:

3.61

Species:

Homo sapiens

PMID:

34009727

Title:

Collective influence of substrate chemistry with physiological fluid shear stress on human umbilical vein endothelial cells

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Endocrinology

Application:

IF:

5.05

Species:

Rattus norvegicus

PMID:

33367902

Title:

Activation of ovarian taste receptors inhibits progesterone production potentially via NO/cGMP and apoptotic signaling

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Homo sapiens

PMID:

35846993

Title:

Icariside Ⅱ Attenuates Palmitic Acid-Induced Endothelial Dysfunction Through SRPK1-Akt-eNOS Signaling Pathway - PMC

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Homo sapiens

PMID:

27590357

Title:

Identification of resveratrol derivative 3, 3′, 4, 4′, 5, 5′-hexamethoxy-trans-stilbene as a novel pro-angiogenic small-molecule compound

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

36016606

Title:

Exploring the pharmacological mechanism of compound kushen injection in the treatment of breast cancer using in vitro experiments: Coupling network pharmacology with GEO database - PMC

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

6.37

Species:

Mus musculus

PMID:

36180828

Title:

miR-351 promotes atherosclerosis in diabetes by inhibiting the ITGB3/PIK3R1/Akt pathway and induces endothelial cell injury and lipid accumulation - PMC

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

PeerJ

Application:

IF:

3.06

Species:

Homo sapiens

PMID:

36312762

Title:

Icariside II induces rapid phosphorylation of endothelial nitric oxide synthase via multiple signaling pathways - PMC

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Journal of Cellular Physiology

Application:

WB, IHC

IF:

6.513

Species:

Homo sapiens

PMID:

30146689

Title:

Astragaloside IV enhances taxol chemosensitivity of breast cancer via caveolin1targeting oxidant damage

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Cell death and disease

Application:

IF:

9.685

Species:

Homo sapiens, Rattus norvegicus

PMID:

29760379

Title:

Autophagic degradation of caveolin-1 promotes liver sinusoidal endothelial cells defenestration

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Current pharmaceutical design

Application:

IF:

3.31

Species:

Mus musculus

PMID:

37881071

Title:

Rosuvastatin Improves Endothelial Dysfunction in Diabetes by Normalizing Endoplasmic Reticulum Stress via Calpain-1 Inhibition

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

EBioMedicine

Application:

IF:

11.205

Species:

Mus musculus

PMID:

27333037

Title:

Enho Mutations Causing Low Adropin: A Possible Pathomechanism of MPO-ANCA Associated Lung Injury.

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.988

Species:

Rattus norvegicus

PMID:

33123008

Title:

Tetramethylpyrazine Ameliorates Lipopolysaccharide-Induced Sepsis in Rats via Protecting Blood-Brain Barrier, Impairing Inflammation and Nitrous Oxide Systems

References for eNOS Rabbit pAb (A1548)

Product:

eNOS Rabbit pAb

Journal:

Molecular Medicine Reports

Application:

IF:

3.423

Species:

Mus musculus

PMID:

33236768

Title:

Bone marrow stem cells therapy alleviates vascular injury in a chronic obstructive pulmonary diseaseobstructive sleep apnea overlap syndrome rat model