제품 > 항체 > 단일클론항체(mAb)

iNOS Rabbit mAb (A3774)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from RAW 264.7 cells using iNOS Rabbit mAb (A3774) at 1:1000 dilution incubated overnight at 4℃. Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - iNOS Rabbit mAb (A3774)

Confocal imaging of RAW 264.7 cells (treated with LPS) and RAW 264.7 cells(untreated) using iNOS Rabbit mAb (A3774, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	iNOS Rabbit mAb
Catalog No.	A3774
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0832

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 2-200 of human iNOS (P35228).
Sequence	ACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRC
Gene ID
Swiss Prot
Synonyms	NOS; INOS; NOS2A; HEP-NOS; iNOS
Calculated MW	131kDa
Observed MW	130kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 IF/ICC 1:200 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	RAW 264.7
Cellular location	cortical cytoskeleton, cytoplasm, cytosol, nucleoplasm, nucleus, perinuclear region of cytoplasm, peroxisomal matrix, peroxisome, plasma membrane.
Customer validation	WB(Mus musculus, Rattus norvegicus, Felis catus, Other) IF(Other, Homo sapiens, Oryctolagus cuniculus, Mus musculus) WB(Other, Mus musculus) IF(Mus musculus) ELISA(Rattus norvegicus) WB (Mus musculus) IHC(Mus musculus) IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구내분비 & 대사Insulin Receptor Signaling Pathway

iNOS Rabbit mAb images

Western blot - iNOS Rabbit mAb (A3774)

Immunofluorescence - iNOS Rabbit mAb (A3774)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Inflammopharmacology

Application:

IF:

5.09

Species:

Mus musculus

PMID:

36401729

Title:

Maltol ameliorates intervertebral disc degeneration through inhibiting PI3K/AKT/NF-κB pathway and regulating NLRP3 inflammasome-mediated pyroptosis

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

36786681

Title:

Confusoside, a dihydrochalcone glucoside, prevents acetaminophen-induced liver injury by modulating the Keap1-Nrf2/Nf-κB/Caspase signaling pathway

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

37142141

Title:

Quercetin serves as the major component of Xiang-lian Pill to ameliorate ulcerative colitis via tipping the balance of STAT1/PPARγ and dictating the alternative …

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

3.32

Species:

Mus musculus

PMID:

37776747

Title:

Copper-containing titanium alloys promote the coupling of osteogenesis and angiogenesis by releasing copper ions

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

European Journal of Pharmacology

Application:

IF:

Species:

Rattus norvegicus

PMID:

38059448

Title:

MiR-223-3p attenuates M1 macrophage polarization via suppressing the Notch signaling pathway and NLRP3-mediated pyroptosis in experimental autoimmune

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.58

Species:

Mus musculus

PMID:

35902863

Title:

Transforming growth factor-1 protects against LPC-induced cognitive deficit by attenuating pyroptosis of microglia via NF-B/ERK1/2 pathways - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Molecular cell

Application:

IF:

19.32

Species:

Mus musculus

PMID:

38266638

Title:

Serine synthesis sustains macrophage IL-1 production via NAD+-dependent protein acetylation

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of virology

Application:

IF, WB

IF:

5.4

Species:

Other

PMID:

38345385

Title:

GCRV-II invades monocytes/macrophages and induces macrophage polarization and apoptosis in tissues to facilitate viral replication and dissemination

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.4

Species:

Mus musculus

PMID:

38380576

Title:

The water-soluble subfraction from Artemisia argyi alleviates LPS-induced inflammatory responses via multiple pathways and targets in vitro and in vivo

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

38342630

Title:

Inflammatory FibroblastLike SynoviocyteDerived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Biomedicines

Application:

IF:

4.7

Species:

Homo sapiens

PMID:

38398043

Title:

Multifunctional Exosomes Derived from M2 Macrophages with Enhanced Odontogenesis, Neurogenesis and Angiogenesis for Regenerative Endodontic Therapy: An In Vitro and In Vivo Investigation - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Chemistry & biodiversity

Application:

IF:

2.9

Species:

Mus musculus

PMID:

37933979

Title:

Dendrocandin U from Dendrobium officinale Kimura et Migo Inhibits M1 Polarization in Alveolar Macrophage by Suppressing NFB Signaling Pathway

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

38468126

Title:

Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR2/SRC pathway in vascular cognitive impairment mice

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Advanced healthcare materials

Application:

WB, IF

IF:

11.09

Species:

Mus musculus

PMID:

35543500

Title:

Low Surface Accessible Area NanoCoral TiO 2 for the Reduction of Foreign Body Reaction During Implantation

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Mus musculus

PMID:

36989784

Title:

Role of Macrophage AHR/TLR4/STAT3 Signaling Axis in the Colitis Induced by Non-canonical AHR ligand Aflatoxin B1

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Communications chemistry

Application:

IF:

7.21

Species:

Mus musculus

PMID:

38167859

Title:

Discovery from Hypericum elatoides and synthesis of hyperelanitriles as -aminopropionitrile-containing polycyclic polyprenylated acylphloroglucinols - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Communications chemistry

Application:

IF:

7.21

Species:

Mus musculus

PMID:

38167859

Title:

Discovery from Hypericum elatoides and synthesis of hyperelanitriles as -aminopropionitrile-containing polycyclic polyprenylated acylphloroglucinols - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

International wound journal

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

38705731

Title:

Combining antibiotic-loaded bone cement-based free vastus lateralis muscle-sparing flap with split-thickness skin grafts: A reliable strategy for reconstructing diabetic foot ulcers at non-weight-bearing areas - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Felis catus

PMID:

38612546

Title:

The Tumor Microenvironment Mediates the HIF-1/PD-L1 Pathway to Promote Immune Escape in Colorectal Cancer - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

International journal of molecular sciences

Application:

WB, ELISA

IF:

6.2

Species:

Rattus norvegicus

PMID:

36902483

Title:

Ceria Nanoparticles Alleviated Osteoarthritis through Attenuating Senescence and Senescence-Associated Secretory Phenotype in Synoviocytes - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Mus musculus

PMID:

36003390

Title:

EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Bioactive materials

Application:

IF:

18.9

Species:

Mus musculus

PMID:

38827172

Title:

Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Rattus norvegicus

PMID:

38970101

Title:

Neuroimmune modulating and energy supporting nanozyme-mimic scaffold synergistically promotes axon regeneration after spinal cord injury - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

7.3

Species:

Mus musculus

PMID:

38803504

Title:

Corilagin alleviates atherosclerosis by inhibiting NLRP3 inflammasome activation via the Olfr2 signaling pathway in vitro and in vivo - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Cardiovascular diabetology

Application:

IF:

9.3

Species:

Mus musculus

PMID:

38702777

Title:

Ion channel Piezo1 activation aggravates the endothelial dysfunction under a high glucose environment - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus,Rattus norvegicus

PMID:

38638567

Title:

Semaphorin3C identified as mediator of neuroinflammation and microglia polarization after spinal cord injury - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.6

Species:

Oryctolagus cuniculus

PMID:

38955026

Title:

Irisin attenuates acute glaucoma-induced neuroinflammation by activating microglia-integrin V5/AMPK and promoting autophagy

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

4.5

Species:

Mus musculus

PMID:

39372592

Title:

Diabetes Mellitus Inhibits Hair Follicle Regeneration by Inducing Macrophage Reprogramming-Mediated Pyroptosis - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

International immunopharmacology

Application:

IHC, WB

IF:

5.6

Species:

Mus musculus

PMID:

38762924

Title:

The Stat3 Inhibitor B9 Alleviates Lipopolysaccharide-Induced Acute Lung Injury Through its Anti-Inflammatory Effects

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Heliyon

Application:

IF, IHC, WB

IF:

Species:

Mus musculus

PMID:

39170188

Title:

Porphyromonas gingivalis promote microglia M1 polarization through the NF-кB signaling pathway - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.2

Species:

Other

PMID:

39410151

Title:

Characterization of the Composition of Bioactive Fractions from Dendrobium officinale Flowers That Protect against H2O2-Induced Oxidative Damage through the PI3K/AKT/Nrf2 Pathway - PMC

References for iNOS Rabbit mAb (A3774)

Product:

iNOS Rabbit mAb

Journal:

Cell biology and toxicology

Application:

IF, WB

IF:

6.1

Species:

Mus musculus

PMID:

39460844

Title:

CTRP9 attenuates peripheral nerve injury-induced mechanical allodynia and thermal hyperalgesia through regulating spinal microglial polarization and neuroinflammation mediated by AdipoR1 in male mice - PMC