제품 > 항체 > 다중클론항체(pAb)

p38 MAPK Rabbit pAb (A0227)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using p38 MAPK Rabbit pAb (A0227) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates using p38 MAPK Rabbit pAb (A0227) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of various lysates using p38 MAPK Rabbit pAb (A0227) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - p38 MAPK Rabbit pAb (A0227)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using p38 MAPK Rabbit pAb (A0227) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung using p38 MAPK Rabbit pAb (A0227) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta using p38 MAPK Rabbit pAb (A0227) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - p38 MAPK Rabbit pAb (A0227)

Immunofluorescence analysis of L929 cells using p38 MAPK Rabbit pAb (A0227) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	p38 MAPK Rabbit pAb
Catalog No.	A0227
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 261-360 of human p38 MAPK (NP_620581.1).
Sequence	SLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES
Gene ID
Swiss Prot
Synonyms	RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; p38 MAPK
Calculated MW	41kDa
Observed MW	38kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T, Jurkat, BxPC-3, RD, U-87MG, LO2, NIH/3T3, Mouse liver, Mouse kidney, Mouse thymus, Mouse brain, Mouse pancreas, Mouse testis, Rat kidney, Rat skeletal muscle
Cellular location	Cytoplasm, Nucleus.
Customer validation	(Raw264.7, HepG2, Mouse) WB(Mus musculus, Homo sapiens, Rattus norvegicus, Danio rerio, Gallus gallus, Mus musculus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

p38 MAPK Rabbit pAb images

Western blot - p38 MAPK Rabbit pAb (A0227)

Immunohistochemistry - p38 MAPK Rabbit pAb (A0227)

Immunofluorescence - p38 MAPK Rabbit pAb (A0227)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0227

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Reviews List

Product Protocols

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

VIRUSES-BASEL

Application:

IF:

5.048

Species:

Mus musculus

PMID:

26053925

Title:

Hydrogen Peroxide Induce Human Cytomegalovirus Replication through the Activation of p38-MAPK Signaling Pathway

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

International Journal Of Clinical and Experimental Pathology

Application:

IF:

0.279

Species:

Homo sapiens

PMID:

23923067

Title:

RAF1-MEK1-ERK/AKT axis may confer NSCLC cell lines resistance to erlotinib

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Toxicology and applied pharmacology

Application:

IF:

4.219

Species:

Homo sapiens

PMID:

28341536

Title:

The role of cPLA2 in Methylglyoxal-induced cell apoptosis of HUVECs

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Neuroscience

Application:

IF:

3.59

Species:

Mus musculus

PMID:

29382550

Title:

Neuroprotective effect of Sirt2-specific inhibitor AK-7 against acute cerebral ischemia is P38 activation-dependent in mice

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

International Journal of Molecular Medicine

Application:

IF:

4.101

Species:

Mus musculus

PMID:

29138800

Title:

Broadleaf Mahonia attenuates granulomatous lobular mastitis?associated inflammation by inhibiting CCL?5 expression in macrophages

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie

Application:

IF:

6.529

Species:

Rattus norvegicus

PMID:

30372831

Title:

Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors.

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Mus musculus

PMID:

33014270

Title:

Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Chemosphere

Application:

IF:

7.086

Species:

Danio rerio

PMID:

33971419

Title:

Benzophenone-3 induced abnormal development of enteric nervous system in zebrafish through MAPK/ERK signaling pathway

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Free Radical Biology and Medicine

Application:

IF:

7.376

Species:

Homo sapiens

PMID:

26456053

Title:

Novel role of TRPV2 in promoting the cytotoxicity of H2O2-mediated oxidative stress in human hepatoma cells

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Phytotherapy research : PTR

Application:

IF:

6.38

Species:

Rattus norvegicus

PMID:

36581492

Title:

Total saponins of panax ginseng via the CX3CL1/CX3CR1 axis attenuates neuroinflammation and exerted antidepressant‐like effects in chronic unpredictable mild …

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

36577154

Title:

Nuciferine alleviates intestinal inflammation by inhibiting MAPK/NF-κB and NLRP3/Caspase 1 pathways in vivo and in vitro

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Veterinary research

Application:

IF:

3.82

Species:

Gallus gallus

PMID:

37337278

Title:

Low let-7d microRNA levels in chick embryos enhance innate immunity against Mycoplasma gallisepticum by suppressing the mitogen-activated protein kinase pathway - PMC

References for p38 MAPK Rabbit pAb (A0227)

Product:

p38 MAPK Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

4.996

Species:

Homo sapiens

PMID:

27586056