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p38 MAPK Rabbit pAb (A14401)

Publications (88) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - p38 MAPK Rabbit pAb (A14401)

Western blot analysis of various lysates using p38 MAPK Rabbit pAb (A14401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - p38 MAPK Rabbit pAb (A14401)

Immunohistochemistry analysis of paraffin-embedded Human placenta using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p38 MAPK Rabbit pAb (A14401)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - p38 MAPK Rabbit pAb (A14401)

Immunofluorescence analysis of NIH-3T3 cells using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - p38 MAPK Rabbit pAb (A14401)

Immunoprecipitation analysis of 200 μg extracts of U-87MG cells, using 3 μg p38 MAPK antibody (A14401). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (A14401) at a dilution of 1:1000.

Overview

Product namep38 MAPK Rabbit pAb
Catalog No.A14401
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 261-360 of human p38 MAPK (NP_620581.1).
SequenceSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES
Gene ID
Swiss Prot
SynonymsRK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; p38 MAPK
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, BxPC-3, Mouse testis, Rat kidney
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Bos taurus, Mus musculus, Other, Sus scrofa, Gallus gallus)

IHC(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - p38 MAPK Rabbit pAb (A14401)}

Western blot - p38 MAPK Rabbit pAb (A14401)

Western blot analysis of various lysates using p38 MAPK Rabbit pAb (A14401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - p38 MAPK Rabbit pAb (A14401)}

Immunohistochemistry - p38 MAPK Rabbit pAb (A14401)

Immunohistochemistry analysis of paraffin-embedded Human placenta using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p38 MAPK Rabbit pAb (A14401)}

Immunohistochemistry - p38 MAPK Rabbit pAb (A14401)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - p38 MAPK Rabbit pAb (A14401)}

Immunofluorescence - p38 MAPK Rabbit pAb (A14401)

Immunofluorescence analysis of NIH-3T3 cells using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - p38 MAPK Rabbit pAb (A14401)}

Immunoprecipitation - p38 MAPK Rabbit pAb (A14401)

Immunoprecipitation analysis of 200 μg extracts of U-87MG cells, using 3 μg p38 MAPK antibody (A14401). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (A14401) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14401? Please let us know so that we can cite the reference in this datasheet.

항체 (8)

Secondary Antibodies (26)