제품 > 항체 > 다중클론항체(pAb)

p53 Rabbit pAb (A3185)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using p53 Rabbit pAb (A3185) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from NIH/3T3 cells, using p53 Rabbit pAb (A3185) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A3185)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using p53 Rabbit pAb (A3185) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of MCF-7 cells using p53 Rabbit pAb (A3185).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Immunofluorescence analysis of HeLa cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using p53 Rabbit pAb (A3185) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A3185)

Immunoprecipitation analysis of 200 μg extracts of U-251MG cells using 3 μg p53 Rabbit pAb (A3185). Western blot was performed from the immunoprecipitate using p53 Rabbit pAb (A3185) at a dilition of 1:1000.

Review

Overview

Product name	p53 Rabbit pAb
Catalog No.	A3185
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-393 of human p53 (NP_000537.3).
Sequence	MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Gene ID
Swiss Prot
Synonyms	P53; BCC7; LFS1; BMFS5; TRP53; p53
Calculated MW	44kDa
Observed MW	53kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	U-251MG, MCF7, 293T, NIH/3T3
Cellular location	Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body.
Customer validation	(U2OS, Non-small-cell carcinoma) WB(Homo sapiens, Sanghuangporus vaninii, Rattus norvegicus, Aedes aegypti, Mus musculus, Other) IHC(Mus musculus) qRT-PCR(Mus musculus) IHC(Mus musculus) IHC(Rattus norvegicus) IF(Rattus norvegicus)

Documents

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Research Area

p53 Rabbit pAb images

Western blot - p53 Rabbit pAb (A3185)

Immunohistochemistry - p53 Rabbit pAb (A3185)

Immunofluorescence - p53 Rabbit pAb (A3185)

Immunofluorescence analysis of MCF-7 cells using p53 Rabbit pAb (A3185).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Immunofluorescence - p53 Rabbit pAb (A3185)

Immunoprecipitation - p53 Rabbit pAb (A3185)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A3185

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Reviews List

Product Protocols

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Acta Pharmacologica Sinica

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

28216618

Title:

1,2:5,6-dianhydrogalactitol inhibits human glioma cell growth in vivo and in vitro by arresting the cell cycle at G2/M phase

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Int J Biol Macromol

Application:

IF:

7.7

Species:

Sanghuangporus vaninii

PMID:

32629056

Title:

Characterization of a polysaccharide from Sanghuangporus vaninii and its antitumor regulation via activation of the p53 signaling pathway in breast cancer MCF-7 …

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Biomolecules

Application:

IF:

5.5

Species:

Rattus norvegicus

PMID:

35327620

Title:

Transplantation of Human Umbilical Cord Mesenchymal Stem Cells-Derived Neural Stem Cells Pretreated with Neuregulin1β Ameliorate Cerebral Ischemic Reperfusion Injury in Rats

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

European journal of medical research

Application:

IHC

IF:

2.8

Species:

Mus musculus

PMID:

36750876

Title:

Research on the mechanism of prednisone in the treatment of ITP via VIP/PACAP-mediated intestinal immune dysfunction - PMC

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Chinese medicine

Application:

qRT-PCR, IHC

IF:

5.3

Species:

Mus musculus

PMID:

36997948

Title:

Protective effects of Qing-Re-Huo-Xue formula on bleomycin-induced pulmonary fibrosis through the p53/IGFBP3 pathway - PMC

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Aedes aegypti

PMID:

37723154

Title:

Aedes aegypti Argonaute 2 controls arbovirus infection and host mortality - PMC

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

6.2

Species:

Homo sapiens, Mus musculus

PMID:

35588622

Title:

Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-B signaling pathway mediated by the DNA damage response

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

6.2

Species:

Homo sapiens, Mus musculus

PMID:

35588622

Title:

Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-B signaling pathway mediated by the DNA damage response

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

6.2

Species:

Homo sapiens, Mus musculus

PMID:

35588622

Title:

Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-B signaling pathway mediated by the DNA damage response

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Cellular and molecular life sciences : CMLS

Application:

IF:

6.2

Species:

Mus musculus

PMID:

38459149

Title:

PRMT5-mediated homologous recombination repair is essential to maintain genomic integrity of neural progenitor cells - PMC

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

WB, IHC, IF

IF:

Species:

Rattus norvegicus

PMID:

37165721

Title:

Extracellular VesicleConjugated Functional Matrix Hydrogels Prevent Senescence by Exosomal miR35945pTargeted HIPK2/p53 Pathway for Disc Regeneration

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.4

Species:

Mus musculus

PMID:

37406788

Title:

Downregulation of a potential therapeutic target NPAS2, regulated by p53, alleviates pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via

References for p53 Rabbit pAb (A3185)

Product:

p53 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Other

PMID:

38933967

Title: