Overview
| Product name | Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling) |
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| Catalog No. | RP02749B |
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| Description | Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling) is produced by Expi293 expression system. The target protein is expressed with sequence (Asp296-Gly580) of Cynomolgus MSLN fused with a His tag at the C-terminal. |
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| Purity | > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Cynomolgus |
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| Calculated MW | 33 kD |
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Background
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Product information
| Synonym | MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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Usage guide
| Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
