Overview
| Product name | Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein |
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| Catalog No. | RP02274B |
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| Description | Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2(275-496) Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Lys275-Phe496) of Human Angiopoietin-2/ANG-2/ANGPT2 fused with a His and Avi tag at the N-terminus. |
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| Purity | > 95% by Tris-Bis PAGE and HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 28.4 kDa |
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Background
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
Product information
| Synonym | AGPT2; ANG2; ANGPT2; ANG2 |
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| Expression Host | HEK293 cells |
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| Tag | N-His&Avi |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
