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Biotinylated Recombinant Human B7-1/CD80 Protein (RP00562B)

Datasheet

Overview

Product nameBiotinylated Recombinant Human B7-1/CD80 Protein
Catalog No.RP00562B
DescriptionBiotinylated Recombinant Human B7-1/CD80 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val35-Asn242) of Human B7-1/CD80 (Accession #P33681-1) fused with a C-His&Avi tag at the C-terminus.
Purity> 95% by SDS-PAGE;> 95% by HPLC
Endotoxin< 1 EU/μg of the protein by LAL method
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
SpeciesHuman
Calculated MW26.8 kDa
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
SynonymCD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
Expression HostHEK293 cells
TagC-His&Avi
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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