Overview
| Product name | Biotinylated Recombinant Human BTLA/CD272 Protein |
|---|
| Catalog No. | RP02572B |
|---|
| Description | Biotinylated Recombinant Human BTLA/CD272 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Lys31-Ser150) of Human BTLA/CD272 fused with His tag and Avi tag at the C-terminus. |
|---|
| Purity | > 95% by Tris-Bis PAGE and HPLC |
|---|
| Endotoxin | < 1 EU/μg of the protein by LAL method. |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation. |
|---|
| Species | Human |
|---|
| Calculated MW | 16.7 kDa |
|---|
Background
B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 . BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
Product information
| Synonym | CD272; BTLA; BTLA1; FLJ16065; MGC129743 |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His&Avi |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
