Overview
| Product name | Biotinylated Recombinant Human CD3E&CD3G (Primary Amine Labeling) |
|---|
| Catalog No. | RP00415B |
|---|
| Description | Biotinylated Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G)) of Human CD3E&CD3G/CD3 epsilon&CD3 gamma (Primary Amine Labeling) (Accession #P07766(CD3E)&P09693(CD3G)) fused with a C-His tag at the C-terminus. |
|---|
| Purity | > 95% by SDS-PAGE；> 95% by HPLC |
|---|
| Endotoxin | < 1 EU/μg of the protein by LAL method |
|---|
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|---|
| Species | Human |
|---|
| Calculated MW | 17.9 kDa (CD3E), 17.9 kDa (CD3G) |
|---|
Background
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product information
| Synonym | CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
