Overview
| Product name | Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein |
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| Catalog No. | RP00630B |
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| Description | Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)) of Human IL-23 alpha&IL-12 beta (Accession #Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)) fused with a C-His&Avi tag at the C-terminus. |
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| Purity | > 95% by SDS-PAGE |
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| Endotoxin | < 1 EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 21.7 kDa (IL-23 alpha) , 34.7 kDa (IL-12 beta) |
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Background
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.
Product information
| Synonym | IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
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| Expression Host | HEK293 cells |
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| Tag | C-His&Avi |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
