Overview
| Product name | Biotinylated Recombinant Human MHC class I polypeptide-related sequence B/MIC-B Protein |
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| Catalog No. | RP02442B |
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| Description | Biotinylated Recombinant Human MHC class I polypeptide-related sequence B/MIC-B Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala23-Gly298) of Human MICB fused with a His and Avi tag at the C-terminal. |
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| Purity | > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 34.4 kDa |
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Background
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Product information
| Synonym | MICB; PERB11.2 |
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| Expression Host | HEK293 cells |
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| Tag | C-His&Avi |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
