Overview
Product name | Biotinylated Recombinant Human WAIF1/5T4/TPBG Protein |
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Catalog No. | RP02602B |
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Description | Biotinylated Recombinant Human WAIF1/5T4/TPBG Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser32-Ser355) of Human TPBG fused with a His and Avi tag at the C-terminal. |
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Purity | > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC |
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Endotoxin | < 1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Human |
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Calculated MW | 38.2 kDa |
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Background
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Product information
Synonym | M6P1; 5T4AG; WAIF1; TPBG; 5T4 |
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Expression Host | HEK293 cells |
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Tag | C-His&Avi |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.