Biotinylated Recombinant Human sTfR/CD71 Protein (RP00372B)

Overview

Product name	Biotinylated Recombinant Human sTfR/CD71 Protein
Catalog No.	RP00372B
Description	Biotinylated Recombinant Human Transferrin R/CD71 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys89-Phe760) of Human Transferrin R/CD71 (Accession #P02786) fused with a N-His&Avi tag at the N-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	77.9 kDa

The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Product information

Synonym	TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR; Transferrin R
Expression Host	HEK293 cells
Tag	N-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.