Biotinylated Recombinant SARS-COV-2 Spike RBD Protein (RP02326)

Product name	Biotinylated Recombinant SARS-COV-2 Spike RBD Protein
Catalog No.	RP02326
Description	Biotinylated Recombinant SARS-COV-2 SARS-COV-2 Spike RBD Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Arg319-Asn532) of SARS-COV-2 SARS-COV-2 Spike RBD fused with His tag and Avi at the C-terminal.
Bio-Activity	Immobilized Human ACE2, His Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated SARS COV-2 Spike RBD, His Tag with the EC₅₀ of 0.097 μg/mL determined by ELISA.
Purity	> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	SARS-COV-2
Calculated MW	27 kDa

Synonym	S protein RBD; Spike glycoprotein Receptor-binding domain; S glycoprotein RBD; Spike protein RBD
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.