Overview
| Product name | Proteinase K |
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| Catalog No. | RP02503LQ |
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| Description | No DNase contamination detected by digestion of λ DNA at 37 ℃ for 6 hours. No RNases contamination detected by digestion of λ DNA at 25℃ for 16 hours. Host genomic DNA/RNA is no residual detected by PCR. |
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| Bio-Activity | Measured by its digestion activity. The activity is >600 U/mL (>32U/mg). One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 µmol of L-tyrosine.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | Please contact us for more information. |
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| Formulation | Supplied as a 0.22 μm filtered solution in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, pH 7.2-7.4. |
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| Species | Tritirachium album |
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Background
Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0 . Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction . Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA .
Product information
| Synonym | Proteinase K |
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| Expression Host | Pichia pastoris |
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| Tag | Tag Free |
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Usage guide
| Storage | This product is stable at 2-8℃ for up to 18 months，and ≤ -20°C for up to 3 years from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
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* For research use only. Not for therapeutic or diagnostic purposes.
