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Proteinase K (High Conc.) (RP02503)

Bioactivityvalidated

Datasheet

ABclonal:Proteinase K (High Conc.)

Overview

Product nameProteinase K (High Conc.)
Catalog No.RP02503
DescriptionNo DNase contamination detected by digestion of λ DNA at 37 ℃ for 6 hours. No RNases contamination detected by digestion of λ DNA at 25℃ for 16 hours. Host genomic DNA/RNA is no residual detected by PCR.
Bio-ActivityMeasured by its digestion activity. The activity is >30U/mg. One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 µmol of L-tyrosine.
Purity> 95% by SDS-PAGE.
EndotoxinPlease contact us for more information.
FormulationLyophilized powder is recommended to dissolve in in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4.
SpeciesTritirachium album
Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0 . Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction . Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA .
SynonymProteinase K
Expression HostPichia pastoris
TagTag Free
StorageStore the lyophilized protein at 2-8℃ for up to 18 months and ≤ -20°C for 3 year.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP02503? Please let us know so that we can cite the reference in this datasheet.

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