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Recombinant Aequorea victoria EGFP Protein (RPT0003)

Publication (1) Datasheet

Overview

Product nameRecombinant Aequorea victoria EGFP Protein
Catalog No.RPT0003
DescriptionRecombinant Aequorea victoria GFP Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys238) of aequorea victoria GFP (Accession #) fused with a 6×His tag at the C-terminus.
Purity> 95% by SDS-PAGE.
Endotoxin<0.1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesAequorea victoria
Calculated MW27.78 kDa
GFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. GFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Enhanced GFP (eGFP) has F64L and S65T mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃.
SynonymGreen fluorescent protein; GFP; EGFP
Expression HostHEK293 cells
TagC-His
Swiss-Prot
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RPT0003? Please let us know so that we can cite the reference in this datasheet.

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