Overview
| Product name | Recombinant Cynomolgus B7-2/CD86 Protein |
|---|
| Catalog No. | RP02634 |
|---|
| Description | Recombinant Cynomolgus B7-2/CD86 Protein is expressed from Expi293 with His tag at the C-terminal.；It contains Tyr26-His240. |
|---|
| Purity | > 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC |
|---|
| Endotoxin | Less than 1EU per μg by the LAL method. |
|---|
| Species | Cynomolgus |
|---|
| Calculated MW | 26.4 kDa |
|---|
Background
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product information
| Synonym | CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2 |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His |
|---|
| Swiss-Prot | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
