Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein (RP00160)

Overview

Product name	Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein
Catalog No.	RP00160
Description	Recombinant Human5'-Nucleotidase/NT5E/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys547) of human 5'-Nucleotidase/CD73 (Accession #NP_002517.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Human 5-Nucleotidase/CD73 at 2 μg/mL (100 μL/well) can bind 5-Nucleotidase/CD73 Rabbit pAb with a linear range of 0.16-2.93 ng/mL. 2.Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >33654 pmol/min/μg. 3.Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >25, 000 pmol/min/μg.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	61.10 kDa

73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The proteins are used as determinants of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries.

Product information

Synonym	NT5E; CALJA; CD73; E5NT; NT; NT5; NTE; eN; eNT
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.