Overview
| Product name | Recombinant Human ABHD4 Protein |
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| Catalog No. | RP03195 |
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| Description | Recombinant Human ABHD4 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Asp342) of Human ABHD4 (Accession #NP_071343.2) fused with a His tag at the N-terminus. |
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| Purity | > 82 % as determined by SDS-PAGE |
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| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
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| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0. |
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| Species | Human |
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| Calculated MW | 40 kDa. |
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Background
Abhydrolase domain containing 4 (ABHD4), also known as alpha/beta-hydrolase 4 (ABH4) , or lyso-N-acylphosphatidylethanolamine lipase, which belongs to the ABHD4/ABHD5 subfamily of peptidase S33 family. Abhydrolase domain containing (ABHD) gene was a small group belongs to alpha/beta hydrolase superfamily. Known members of this group are all found to be involved in important biochemical processes and related to various diseases. The alpha/beta-hydrolase 4 (ABH4) is a lysophospholipase/phospholipase B that selectively hydrolyzes N-acyl phosphatidylethanolamines (NAPEs) and lysoNAPEs. ABH4 accepts lysoNAPEs bearing both saturated and polyunsaturated N-acyl chains as substrates and displays a distribution that closely mirrors lysoNAPE-lipase activity in mouse tissues. The existence of an NAPE-PLD-independent route for NAE biosynthesis and suggest that ABH4 plays a role in this metabolic pathway by acting as a (lyso)NAPE-selective lipase.
Product information
| Synonym | ABHD4; (Lyso)-N-acylphosphatidylethanolamine lipase; 3.1.1.-; Alpha/beta hydrolase domain-containing protein 4; Abhydrolase domain-containing protein 4; Alpha/beta-hydrolase 4 |
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| Expression Host | Baculovirus-Insect Cells |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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* For research use only. Not for therapeutic or diagnostic purposes.
