Overview
Product name | Recombinant Human ACE/CD143 Protein |
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Catalog No. | RP01863 |
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Description | Recombinant Human ACE Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu30-Leu1261) of Human ACE (Accession #NP_000780.1) fused with a His tag at the N-terminus. |
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Purity | > 92% by SDS-PAGE. |
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Endotoxin | <0.1EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Human |
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Calculated MW | 142.60 kDa |
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Background
Angiotensin I-converting enzyme (ACE, CD143) hydrolyzes small peptides such as angiotensin I, bradykinin, substance P, LH-RH and several others and thus plays a key role in blood pressure regulation and vascular remodeling. ACE plays a crucial role in blood pressure regulation, vascular remodeling, and immunity.ACE is a Zn2+ carboxydipeptidase which plays a key role in the regulation of blood pressure and also in the development of vascular pathologies and tissue remodeling. ACE is expressed as two isoforms: somatic ACE (sACE), which is responsible for its hypertensive properties, and a smaller isoform (testicular ACE), which is expressed solely in germinal cells.
Product information
Synonym | ACE; DCP; DCP1,Angiotensin-converting enzyme; ACE; 3.4.15.1; Dipeptidyl carboxypeptidase I; Kininase II; CD143; Cleaved into: Angiotensin-converting enzyme; soluble form. |
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Expression Host | HEK293 cells |
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Tag | N-his |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.