Overview
| Product name | Recombinant Human Aldehyde reductase/AKR1B1 Protein |
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| Catalog No. | RP02938 |
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| Description | Recombinant Human Aldehyde reductase/AKR1B1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Phe316) of human Aldehyde reductase/AKR1B1 (Accession #NP_001619.1) fused with 6×His tag at the N-terminus. |
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| Purity | > 90 % as determined by SDS-PAGE |
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| Endotoxin | Please contact us for more information. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, 20% glycerol, pH 7.5. |
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| Species | Human |
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| Calculated MW | 37.9 kDa |
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Background
Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.
Product information
| Synonym | AKR1B1; ADR; ALDR1; ALR2; AR; aldose reductase; ADR; ALDR1; ALR2; AR |
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| Expression Host | E. coli |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
