Overview
| Product name | Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein |
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| Catalog No. | RP03154 |
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| Description | Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Asp502) of human ALPG (Accession #NP_112603) fused with a His tag at the C-terminus. |
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| Purity | ≥ 95 % as determined by SDS-PAGE |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 53.28 kDa |
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Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product information
| Synonym | ALPG; ALPPL; ALPPL2; Alkaline phosphatase; germ cell type; EC:3.1.3.1; ALP-1; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase; placental-like; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-like |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
