Overview
| Product name | Recombinant Human Alkaline phosphatase (Intestinal type)/ALPI Protein |
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| Catalog No. | RP02744 |
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| Description | Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Val20-Asp503) of Human Alkaline phosphatase (Intestinal type) /ALPI (Accession # P09923) fused with His tag and Flag tag at the N-terminus. |
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| Purity | > 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC |
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| Endotoxin | Less than 1EU per μg by the LAL method. |
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| Species | Human |
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| Calculated MW | 54.5 kDa |
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Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product information
| Synonym | ALPI，Intestinal-type alkaline phosphatase; IAP; Intestinal alkaline phosphatase; |
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| Expression Host | HEK293 cells |
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| Tag | N-His&Flag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
