Overview
| Product name | Recombinant Human Alkaline phosphatase (Placental type) /ALPP Protein |
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| Catalog No. | RP03155 |
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| Description | Recombinant Human Alkaline phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile23-Asp506. |
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| Bio-Activity | Immobilized Human Alkaline Phosphatase (Placental type) , His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 11.1 ng/mL determined by ELISA.
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| Purity | ≥ 95 % as determined by SDS-PAGE |
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.2mM CaCl2, pH 7.5. |
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| Species | Human |
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| Calculated MW | 53.8 kDa |
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Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product information
| Synonym | ALPP; PLAP; Alkaline phosphatase; placental type; EC:3.1.3.1; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1 |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
