Overview
Product name | Recombinant Human Aminoacylase 1/ACY 1 Protein |
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Catalog No. | RP03197 |
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Description | Recombinant Human Aminoacylase 1/ACY 1 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Ser408) of Human Aminoacylase 1/ACY 1 (Accession #NP_000657.1) fused with a His tag at the C-terminus. |
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Purity | > 95 % as determined by SDS-PAGE |
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Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
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Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol |
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Species | Human |
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Calculated MW | 47.3 kDa |
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Background
Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Product information
Synonym | ACY1,Aminoacylase-1; ACY-1; 3.5.1.14; N-acyl-L-amino-acid amidohydrolase |
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Expression Host | Baculovirus-Insect Cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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* For research use only. Not for therapeutic or diagnostic purposes.