Overview
| Product name | Recombinant Human Amphiregulin/AREG Protein |
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| Catalog No. | RP00898 |
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| Description | Recombinant Human Amphiregulin/AREG Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser101-Lys198) of Human Amphiregulin/AREG (Accession #P15514) fused with an initial Met at the N-terminus. |
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| Bio-Activity | Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-15 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 11.4 kDa |
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Product information
| Synonym | AREG; AR; AREGB; CRDGF; SDGF; amphiregulin |
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| Expression Host | E. coli |
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| Tag | No tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
