Overview
| Product name | Recombinant Human Angiopoietin-like 7/ANGPTL7(27-346) Protein |
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| Catalog No. | RP00206 |
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| Description | Recombinant Human Angiopoietin-like 7/ANGPTL7(27-346) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln27-Pro346) of human Angiopoietin-like 7 (Accession #NP_066969.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ANGPTL7 at 5 μg/mL (100 μL/well) can bind Recombinant Human LILRB4 with a linear range of 0.5-3.5 μg/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 37.90 kDa |
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Background
Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Its production is up regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans . When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis.
Product information
| Synonym | ANGPTL7; AngX; CDT6; dJ647M16.1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
