Overview
| Product name | Recombinant Human Annexin A2/ANXA2 Protein |
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| Catalog No. | RP02731 |
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| Description | Recombinant Human Annexin A2/ANXA2 Protein is produced by E. coli expression system. The target protein is expressed with sequence Ser2-Asp339. of Human Annexin A2/ANXA2 (Accession #) fused with His tag at the N-terminal |
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| Purity | > 95% as determined by Tris-Bis PAGE |
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| Endotoxin | Less than 1EU per μg by the LAL method. |
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| Species | Human |
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| Calculated MW | 39.6 kDa |
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Background
ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.
Product information
| Synonym | Chromobindin-8; Lipocortin II; PAP-IV; ANX2; ANX2L4; CAL1H; LPC2D; ANXA2; Annexin A2; Annexin II; LIP2PAP-IV; LPC2D; LPC2DP36; p36 |
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| Expression Host | E. coli |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
