Overview
| Product name | Recombinant Human ApolipoproteinA-IV/APOA4 Protein |
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| Catalog No. | RP01854 |
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| Description | Recombinant Human Apolipoprotein A-IV/APOA4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu21-Ser396) ofHuman Apolipoprotein A-IV/APOA4 (Accession #NP_000473.2) fused with a His tag at the C-terminus. |
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| Endotoxin | <0.01EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 44.22 kDa |
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Background
Apolipoprotein A-IV (ApoA-IV), first discovered in rat plasma in the 1970s , is a 46 kD glycoprotein with relatively high concentrations in plasma (15–37 mg/dL) . It is expressed predominantly in the mammalian small intestine (with highest expression in the duodenum), and also expressed in rodent liver at lower levels. Sensing lipid absorption in enterocytes , ApoA-IV is synthesized and packaged with lipids and other apolipoproteins into chylomicrons and secreted through the lymphatic system into the blood. Once there, as a member of exchangeable apolipoproteins, ApoA-IV dissociates from chylomicrons and transfers to HDL (high density lipoprotein) particles or circulates in lipid-free form .ApoA-IV may protect against atherosclerosis , mediate reverse cholesterol transport , act as an anti-inflammatory agent and modulate intestinal lipid absorption .
Product information
| Synonym | APOA4，Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4. |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
