| Product name | Recombinant Human Argonaute-1/AGO1 Protein |
|---|---|
| Catalog No. | RP02933 |
| Description | Recombinant Human Argonaute-1/AGO1 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Ala857) of human Argonaute-1/AGO1 (Accession #NP_036331.1) fused with a 6×His tag at the N-terminus. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0. |
| Species | Human |
| Calculated MW | 99.5 kDa |
| Synonym | Q99; EIF2C; hAgo1; EIF2C1; GERP95; NEDLBAS; AGO1 |
|---|---|
| Expression Host | Baculovirus-Insect Cells |
| Tag | N-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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