Overview
Product name | Recombinant Human Autotaxin/E-NPP2 Protein |
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Catalog No. | RP02143 |
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Description | Recombinant Human Autotaxin/E-NPP2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp49-Ile863) of human ENPP-2 (Accession #AAH34961.1) fused with a His tag at the N-terminus. |
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Bio-Activity | Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >15000 pmol/min/μg, as measured under the described conditions.
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Purity | > 90% by SDS-PAGE. |
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Endotoxin | < 1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 94.54 kDa |
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Background
ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain.Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.
Product information
Synonym | ENPP2; ATX; ATX-X; AUTOTAXIN; LysoPLD; NPP2; PD-IALPHA; PDNP2 |
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Expression Host | HEK293 cells |
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Tag | N-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.