Recombinant Human B7-1/CD80 Protein (RP00065)

Overview

Product name	Recombinant Human B7-1/CD80 Protein
Catalog No.	RP00065
Description	Recombinant Human B7-1/CD80 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Val35-Asn242) of human B7-1/CD80 (Accession #NP_005182.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant human CD80 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA-4 with a linear range of 10-50 ng/mL.
Purity	> 92% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	24.71 kDa

The protein is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Product information

Synonym	B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; CD80
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.