Overview
| Product name | Recombinant Human B7-H5/Gi24/VISTA Protein |
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| Catalog No. | RP00304 |
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| Description | Recombinant Human B7-H5/Gi24/VISTA Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe33-Ala194) of Human B7-H5/Gi24/VISTA (Accession #Q9H7M9) fused with a C-His tag at the C-terminus. |
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| Purity | > 95% by SDS-PAGE；> 95% by HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 19 kDa |
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Background
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
Product information
| Synonym | B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
