Overview
| Product name | Recombinant Human B7-H6/NCR3LG1 Protein |
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| Catalog No. | RP01356 |
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| Description | Recombinant Human B7-H6/NCR3LG1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp25-Ser262) of human B7-H6/NCR3LG1 (Accession #NP_001189368.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Human NCR3LG1 (Catalog: RP01356) at 2 μg/mL (100 μL/well) can bind Mouse NCR3 (Catalog: RP00179) with a linear range of 0.03-46 ng/mL.
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| Purity | > 90% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 27.51 kDa |
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Background
Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.
Product information
| Synonym | B7-H6; NCR3LG1; B7 Homolog 6; NCR3LG1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
