Overview
| Product name | Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein |
|---|
| Catalog No. | RP00703 |
|---|
| Description | Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His27-Pro104) of human Brain Natriuretic Peptide/BNP (Accession #P16860) fused with a hFC tag at the C-terminus. |
|---|
| Purity | > 95% by SDS-PAGE. |
|---|
| Endotoxin | < 0.01 EU/μg |
|---|
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
|---|
| Species | Human |
|---|
| Calculated MW | 34.60 kDa |
|---|
Background
Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly byventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNPprecursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriureticpeptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from theventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF)and atrial septal defect in patients without HF.
Product information
| Synonym | NPPB; BNP |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-hFC |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
