Overview
| Product name | Recombinant Human CCL14/HCC-1/HCC-3(28-93) Protein |
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| Catalog No. | RP01985 |
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| Description | Recombinant Human CCL14/HCC-1/HCC-3(28-93) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly28-Asn93) of Human CCL14/HCC-1/HCC-3(28-93) (Accession #NP_116739.1) fused with His at the C-terminus. |
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| Purity | ≥ 95 % as determined by SDS-PAGE |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 8.623 KDa |
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Background
Chemokine (C-C motif) Ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It isproduced as a protein precursor that is processed to generate a mature active protein containing 74 aminoacids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed invarious tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does notinduce their chemotaxis. Human CCL14 is located on chromosome 17 within a cluster of other chemokinesbelonging to the CC family.
Product information
| Synonym | CCL14; NCC2; SCYA14; C-C motif chemokine 14; Chemokine CC-1/CC-3; HCC-1/HCC-3; HCC-1(1-74); NCC-2; Small-inducible cytokine A14; Cleaved into: HCC-1(3-74); HCC-1(4-74); HCC-1(9-74) |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
