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Recombinant Human CD19 Protein (RP01307)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human CD19 Protein
Catalog No.RP01307
DescriptionRecombinant Human CD19 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro20-Lys291) of human CD19 (Accession #NP_001761.3) fused with an 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA.Immobilized Human CD19 at 2 μg/mL (100 μL/well) can bind CD19 Rabbit pAb with a linear range of 0.12-3.53ng/mL.
Purity> 90% by SDS-PAGE.
EndotoxinPlease contact us for more information.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesHuman
Calculated MW30.95 kDa
B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to b
SynonymB4; CVID3; CD19
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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