Recombinant Human CD38 Protein (RP00151)

Overview

Product name	Recombinant Human CD38 Protein
Catalog No.	RP00151
Description	Recombinant Human CD38 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val43-Ile300) of human CD38 (Accession #NP_001766.2) fused with a 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Human CD38 Protein at 1μg/mL (100 μL/well) can bind CD38 Rabbit pAb with a linear range of 0.244-3.2 ng/mL. 2.Loaded CD38 Rabbit mAb (Catalog:A20215) on ProA Biosensor, can bind Human CD38 Protein, C-His (Catalog: RP00151) with an affinity constant of 4.1 nM as determined in BLI assay (Gator).
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	30.71 kDa

The protein is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia.

Product information

Synonym	CD38; ADPRC 1; ADPRC1
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.