Overview
| Product name | Recombinant Human CD3E&CD3D Protein |
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| Catalog No. | RP00623 |
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| Description | Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)) of Human CD3E&CD3D/CD3 epsilon&CD3 delta (Accession #P07766(CD3E)&P04234(CD3D)) fused with a C-His tag at the C-terminus. |
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| Purity | > 95% by SDS-PAGE；> 95% by HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 17.9 kDa (CD3E), 16.7 kDa (CD3D) |
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Background
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product information
| Synonym | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
