Overview
| Product name | Recombinant Human CDH3/P-cadherin Protein |
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| Catalog No. | RP01491 |
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| Description | Recombinant Human CDH3/P-cadherin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp108-Gly654) of human CDH3/Cadherin-3 (Accession #NP_001784.2) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1×105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 50-60% will adhere after 1.5 hours at 37° C.
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| Purity | > 85% by SDS-PAGE. |
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| Endotoxin | <1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 60.86 kDa |
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Background
Cadherin-3 （also known as P-cadherin）is a classical cell-to-cell adhesion molecule with a homeostatic function in several normal tissues. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. In humans, cadherin-3 is only detected in a few organs. However, its overexpression is strongly associated with a poor prognosis in some solid tumors including breast, lung and pancreatic cancers.
Product information
| Synonym | CDH3; CDHP; HJMD; PCAD |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
