Overview
Product name | Recombinant Human CISD1 Protein |
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Catalog No. | RP02993 |
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Description | Recombinant Human CISD1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Lys32-Thr108) of human CISD1 (Accession #NP_060934.1) fused with a 6×His tag at the N-terminus. |
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Purity | >90% as determined by SDS-PAGE |
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Endotoxin | Please contact us for more information. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 10 % glycerol. |
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Species | Human |
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Calculated MW | 11.3 kDa |
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Background
Mitochondrial dysfunction is thought to play a significant role in neurodegeneration observed in Parkinson's disease (PD), the loss of mitoNEET (CISD1), an iron-sulfur containing protein that regulates mitochondrial bioenergetics, results in mitochondrial dysfunction and loss of striatal dopamine and tyrosine hydroxylase. CDGSH iron sulfur domain 1 (CISD1, also termed mitoNEET), an iron-containing outer mitochondrial membrane protein, negatively regulates ferroptotic cancer cell death. At the cellular level, CISD1 gene expression increased during human adipocyte differentiation in correlation with adipogenic genes.Thus it is a possible role of CISD1 in obesity-associated dysfunctional adipogenesis in human VAT.
Product information
Synonym | C10orf70; MDS029; mitoNEET; ZCD1 |
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Expression Host | E. coli |
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Tag | N-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.